Plasmid相关论文
...
Transconjugation and genotyping of the plasmid-mediated AmpC β-lactamase and extended-spectrum β-lac
...
目的 构建并鉴定细粒棘球绦虫(Eg)转基因植物载体重组pBI-Eg95质粒.方法 从细粒棘球蚴包囊中分离原头节.经超声粉碎后抽提总RNA,反......
...
Objective: To construct a prokaryotic recombinant vector for mouse lactate dehydrogenase-C and to detect its expression ......
AIM: Toll-like receptor 4 (TLR4) has been shown to be important for bacterial infection, especially to lipopolysaccharid......
Amyloid precursor protein (APP) plays a critical role in the formation of Alzheimer’s disease (AD). The intracellular d......
目的运用水压法复制HBV小鼠急性模型,用于研究双表达siRNA在小鼠体内对HBV复制的抑制作用。方法使用HBV质粒DNA对BALB/c小鼠进行尾......
Recent studies indicate that cell-cycle checkpoints are tightly correlated with the regulation of apoptosis, in which p5......
Objective:Despite platinum-based adjuvant chemotherapy has improved greatly patients’ outcomes,drug resistance poses a ......
AIM:To confirm the role of sex-determining region Y-box 7(Sox7) in aspirin-mediated growth inhibition of COX-independent......
Gene therapy is one of the most attractive fields in tumor therapy. In past decades, significant progress has been achie......
目的构建人7型腺病毒（human adenovirus type 7，HAdV7）检测细胞系。方法将HAdV7中的Hexon基因克隆至带有绿色荧光蛋白基因的慢病毒表......
目的研究克雷伯菌属对亚胺培南耐药机制以及KPC-2基因在克雷伯菌属中传播机制。方法收集四川大学华西医院两个阶段(2009~2010年和2......
该文从挂篮荷载计算、施工流程、支座及临时固结施工、挂篮安装及试验、合拢段施工、模板制作安装、钢筋安装、混凝土的浇筑及养生......
目的 分析我国肠出血性大肠埃希菌 O157∶H7暴发菌株携带的新接合质粒 pO157_Sal的序列特征。方法对我国不同来源携带 pO157_Sal ......
该文从挂篮荷载计算、施工流程、支座及临时固结施工、挂篮安装及试验、合拢段施工、模板制作安装、钢筋安装、混凝土的浇筑及养生......
Transfection Efficiency Comparison of Oligonucleotide and Plasmid to the HL-60 Cell Line with Liposo
The transfection efficiency of oligonucleotide and plasmid to the HL-60 cell line with lipofectaminePLUS was compared th......
Effect of p27Kipl Inhibition on Proliferation of Bovine Corneal Endothelial Cells by RNA Interferenc
Three plasmids (pGenesil-P1, pGenesil-P2, pGenesil-P3) with different p27Kipl-shRNA sequences were designed and synthesi......
背景 研究表明,Krüppel样因子6(KLF6)与生长发育、细胞分化、增生、凋亡、血管生成及组织修复等生理或病理过程有关.在眼科领域,......
Transconjugation and genotyping of the plasmid-mediated AmpC β-lactamase and extended-spectrum β-lac
...
Transfect bone marrow stromal cells with pcDNA3.1-VEGF to construct tissue engineered bone in defect
Background We previously showed that nano-hydroxyapatite/carboxymethyl chitosan (n-Ha/CMCS) displayed excellent mechani......
目的 体外构建含有SCN3A基因mRNA片段的质粒pCMV6-XL4-SCN3A的突变体N302S(c.905A＞G),为进一步的功能研究奠定实验基础.方法 设计带......
目的 在包含腺病毒全基因组40 kb的大质粒上快速实现单碱基突变.方法 通过重叠PCR方法扩增出含有突变位点的打靶DNA,将腺病毒大质......
目的 制备编码人类WT1基因的shRNA(short hairpin RNA)质粒表达载体.方法 设计合成WT1shRNA的DNA模板单链,在两端加入限制性内切酶......
新德里金属β-内酰胺酶(NDM-1)是近来发现的由新的超级耐药基因编码的一种耐药酶.编码该酶的基冈位于质粒等町移动遗传因子上,使得......
目的 通过检测移植经干扰乙酰化作用基因Gen5表达重组质粒ZJ3转染间充质干细胞(mesenchymal stem cells,MSCs)的Wistar大鼠心肌组......
目的 了解温州地区环丙沙星耐药肠杆菌科细菌临床株的qnr、aac(6)-Ⅰ b-cr基因的分布情况.方法 收集2005年8月-2008年4月间温州医......
目的 构建AFP增强子调控的小干扰RNA质粒载体.方法 从HePG2细胞中提取AFP基因.采用定向克隆的方法,构建质粒pGenesil-AFP.pGenesil......
To study the inhibitory effect of Nogo-A shRNA on cell line PC12, the Nogo-A shRNA (short hairpin RNA, or shRNA) was des......
目的 分析沙眼衣原体(Chlamydia trachomatis,Ct)隐蔽性质粒编码的质粒蛋白pORF5在感染细胞中的定位并初步探讨其生物学特征.方法 ......
目的 通过对来自不同年份大肠杆菌质粒DNA序列进行高通最测序,从质粒基因组水平分析大肠杆菌质粒DNA所携带的解离后致死基因的种类......
多重耐药大肠埃希菌(multidrug-resistant Escherichia coli,MDR-ECO)在医院感染病原菌中占重要地位.先前,我们已报道了MDR-ECO老......
目的构建人Smad 4基因质粒体并检测其在胶质瘤细胞系U251内的表达水平及作用。方法构建PEGFP-Smad4质粒体,应用FUGENE HD转染质粒......
目的构建转录因子激活蛋白-2α(transcription factor AP-2 alpha,TFAP2α)重组真核质粒及其稳转细胞株,研究过表达TFAP2α对肾透......
目的 构建MT1F基因过表达重组质粒,并转染结肠癌细胞株.方法 Real-time聚合酶链反应(PCR)检测和筛选6株结肠癌细胞MT1F mRNA表达.......
目的 研究一种质粒DNA荧光标记的简便方法,以便进行DNA递送的示踪.方法 质粒经 溴激活后,于不同温度下存放不同时间,再与1,10-......
Transfect bone marrow stromal cells with pcDNA3.1-VEGF to construct tissue engineered bone in defect
Background We previously showed that nano-hydroxyapatite/carboxymethyl chitosan (n-Ha/CMCS) displayed excellent mechani......
Complete Sequence of pABTJ2, A Plasmid from Acinetobacter baumannii MDR-TJ, Carrying Many Phage-like
Acinetobacter baumannii is an important opportunistic pathogen in hospital, and the multidrug-resistant isolates of A. b......
单增李斯特菌是一种可引起李斯特菌病的重要食源性病原体,质粒在单增李斯特菌中分布广泛,这些质粒对宿主的环境适应能力、毒力以及......
